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. Author manuscript; available in PMC: 2014 Apr 5.
Published in final edited form as: J Proteome Res. 2013 Mar 15;12(4):1789–1796. doi: 10.1021/pr3011342

Figure 4. Identification of the 47-kDa liver fibrosis-associated protein by immunoproteomics.

Figure 4

(A) The 2DE protein profile of HepG2 cells. Lane 1, HepG2 cell lysate; lane 2, protein molecular marker. (B) Western blotting analysis of 2DE gel was probed with a high-titer liver fibrosis serum which contains antibodies to the 47-kDa protein. (C) and (D) The corresponding area (rectangle) of A and B was enlarged. Positive spots were picked up by using PDQUEST software.