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. Author manuscript; available in PMC: 2013 Aug 15.
Published in final edited form as: Leukemia. 2010 Jun 24;24(9):1555–1565. doi: 10.1038/leu.2010.142

Figure 6. Effects of caspase inhibition and iron supplementation on the cytotoxicity of HXR9 and its combination with ch128.1Av.

Figure 6

IM-9 cells were treated with 2.5 nM ch128.1Av, 20 µM HXR9, or their combination for 48 hours; pre-treated with 50 µM ZVAD for 1 hour followed by the above treatments; or treated simultaneously with 25 µM FAC and the above treatments. A) [3H]-thymidine incorporation was used to monitor proliferation. Data are shown as the percent of control cells and represent the mean of triplicate samples. Error bars represent the standard deviation (* p < 0.05, ** p < 0.001). B) Apoptosis in cells treated as in A) was measured by flow cytometry analysis of cells stained with Annexin V and counterstained with PI after 48 hours. Percentage of cells is shown in the corner of each quadrant. Results are representative of two independent experiments.