TABLE 1.
Human colonic isolates examined in this studya
| Strain | 16SrRNA sequence relationship (% identity BlastN) for:
|
Isolation mediumc | SCFA concn (mM) ofd:
|
Reference | ||
|---|---|---|---|---|---|---|
| Closest speciesb | Best match | But. | Ac. | |||
| A2-194 | Roseburia intestinalis (94%) | Clone HuCB37 (99%) | M2GSC | 7.3 | −3.5 | 5 |
| B16/4 | B. fibrisolvens | Closest species | NSM | 18.8 | −3.9 | 42 |
| M72/1 | Roseburia intestinalis (97%) | Clone HuCA13 (99%) | M2WS | 13.9 | −5.4 | This study |
| M6/1 | Roseburia intestinalis (97%) | Clone HuCA13 (99%) | M2GSC | 17.0 | −3.6 | This study |
| M88/1 | Roseburia intestinalis (97%) | Clone HuCA13 (99%) | M2WS | 24.0 | −8.3 | This study |
| M91/1 | Roseburia intestinalis (97%) | Clone HuCA13 (99%) | M2WS | 23.6 | −7.4 | This study |
| M101/3 | Roseburia intestinalis (97%) | Clone HuCA13 (99%) | M2CS | 18.6 | −1.6 | This study |
| A2-183 | Roseburia intestinalis (97%) | Clone adhufec225 (99%) | M2GSC | 20.0 | −8.2 | 5, 18 |
| A2-181 | Roseburia intestinalis (96%) | Strain A2-183 (98%) | M2GSC | 16.8 | −11.5 | 4, 18 |
| L1-82 | Roseburia intestinalis | Closest species | M2GSC | 18.5 | −9.1 | 4, 18, 19 |
| L1-952 | Roseburia intestinalis (99%) | Closest species | M2GSC | 21.3 | −10.0 | 5, 18, 19 |
| L1-8151 | Roseburia intestinalis | Closest species | M2GSC | 16.0 | −12.3 | 4, 18, 19 |
| M50/1 | Roseburia intestinalis (99%) | Strain L1-93 (99%) | M2GSC | 17.5 | −10.9 | This study |
| T1-815 | E. rectale (99%) | Strain A1-86 (99%) | M2GSC | 5.3 | 0.8 | 5 |
| A1-86 | E. rectale (99%) | Closest species | M2GSC | 21.7 | −9.3 | 5 |
| M104/1 | E. rectale (99%) | Strain A1-86 (99%) | M2WS | 10.8 | 1.1 | This study |
| A2-232 | C. nexile (98%) | Strain A2-231 (99%) | M2GSC | 3.8 | 2.1 | 4 |
| SL7/1 | C. nexile (98%) | Strain A2-231 (99%) | M2L | 4.2 | 2.1 | This study |
| M62/1 | C. saccharolyticum (95%) | Closest species | M2GSC | 9.7 | 4.8 | This study |
| SM4/1 | C. sp. DR7 (95%) | Closest species | M2M | 3.6 | −1.0 | This study |
| SS3/4 | C. hathewayi (91%) | Clone HuCA19 (94%) | SS | 13.7 | 10.3 | This study |
| GM2/1 | Ruminococcus gnavus (92%) | Clone HuCA19 (94%) | M2M | 10.6 | 2.3 | This study |
| SR1/1 | Ruminococcus obeum (95%) | Clone HuCB12 (97%) | SR | 5.5 | 18.6 | This study |
| SR1/5 | Ruminococcus obeum (94%) | Clone HuCB12 (97%) | SR | ND | ND | This study |
| SSC/2 | C. indolis (94%) | Clone adhufec25 (99%) | SS | 22.8 | 0.1 | This study |
| SS2/1 | C. indolis (94%) | Clone adhufec25 (99%) | SS | 9.4 | −7.0 | This study |
| L1-92 | A. caccae | Closest species | M2GSC | 20.4 | −16.9 | 5, 44 |
| SM6/1 | E. hallii (98%) | Clone p-2001-s959-5 (99%) | M2M | 14.9 | −1.4 | This study |
| L2-7 | E. hallii (96%) | CloneHuCA15 (98%) | M2GSC | 11.9 | 2.9 | 5 |
| SL6/1/1 | E. hallii (97%) | CloneHuCA15 (99%) | M2L | 11.0 | −4.4 | This study |
| ART55/1 | E. ruminantium (92%) | Clone A19 (99%) | SSL | 1.9 | 0.7 | This study |
| L2-50 | E. ruminantium (93%) | Clone A19 (96%) | M2GSC | 6.2 | 3.1 | 5, 18 |
| T2-87 | E. cylindroides (99%) | Closest species | M2GSC | 5.7 | 2.3 | 4 |
| SM7/11 | E. cylindroides (99%) | Closest species | M2M | 6.0 | 0.3 | This study |
| A2-165 | F. prausnitzii | Closest species | M2GSC | 14.4 | −7.4 | 5, 18, 20 |
| M21/2 | F. prausnitzii (99%) | Closest species | M2CS | 16.0 | −10.0 | This study |
| SL3/3 | F. prausnitzii (98%) | Strain A2-165 (98%) | M2L | 19.5 | −14.5 | This study |
| SL4/4 | F. prausnitzii (98%) | Closest species | M2L | 17.6 | −9.4 | This study |
a
Shown are data for 16S rRNA relationships, isolation media, and net production and utilization of butyrate and acetate.
b
A., Anaerostipes; B., Butyrivibrio; C., Clostridium; E., Eubacterium; F., Faecalibacterium.
c
M2GSC (31); M2CS, M2 (24) plus corn starch; M2WS, M2 plus wheat starch; M2M, M2 plus mannitol; M2L, M2 plus lactate; NSM (42); SS, Selenomonas selective medium; SR, Selenomonas ruminantium medium (2).
d
SCFA, short-chain fatty acid; But, butyrate; Ac, acetate; ND, not determined.