TABLE 1.
B. fungorum LB400 strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| B. fungorum LB400 | Wild type | 3 |
| CM359.1 | ΔmtdB | This study |
| CM359K.1 | ΔmtdB::kan | This study |
| CM359-363.1 | ΔmtdB ΔflhA | This study |
| CM359-363K.1 | ΔmtdB ΔflhA::kan | This study |
| CM359-363-409K.1 | ΔmtdB ΔflhA ΔfdhA::kan | This study |
| CM359-409K.1 | ΔmtdB ΔfdhA::kan | This study |
| CM363.1 | ΔflhA | 19 |
| CM363K.1 | ΔflhA::kan | 19 |
| CM363-409K.1 | ΔflhA ΔfdhA::kan | This study |
| CM409K.1 | ΔfdhA::kan | This study |
| Plasmids | ||
| pCM157 | Cre expression plasmid | 19 |
| pCM184 | Allelic-exchange vector | 19 |
| pCM356 | pCR2.1 with mtdB upstream | This study |
| pCM357 | pCR2.1 with mtdB downstream | This study |
| pCM358 | pCM184 with mtdB downstream | This study |
| pCM359 | pCM358 with mtdB upstream | This study |
| pCM363 | Construct for ΔflhA::kan mutations | 19 |
| pCM406 | pCR2.1 with fdhA upstream | This study |
| pCM407 | pCR2.1 with fdhA downstream | This study |
| pCM408 | pCM184 with fdhA upstream | This study |
| pCM409 | pCM408 with fdhA downstream | This study |
| pCR2.1 | PCR cloning vector | Invitrogen |