Table 1.
E. coli K-12 strains
| Strain | Description | Reference or construction |
|---|---|---|
| BW229 | lac rpsL rfa-209∷Tn10 pyrE70 gltS10 metB thi | E. coli Genetic Stock Center |
| ES1481 | mutS215∷Tn10 | E. coli Genetic Stock Center |
| FC29 | Δ(lac-proAB)XIIIthi ara[F′ proAB+ Δ(lacI-lacZ)] | |
| FC40 | Δ(lac-proAB)XIIIthi ara RifR [F′ proAB+lacI33ΩlacZ] | (14) |
| FC526 | FC40 ΔrecG263∷kan | (17) |
| FC722 | FC40 [F′ zzf-1831∷Tn10dtet+1] | (39) |
| GM2159 | dam13∷Tn9 | M. Marinus (Univ. of Massachusetts, Worcester, MA) |
| GS1481 | ΔruvC64∷kan | (44) |
| SMR749 | FC40 mutS215∷Tn10 | FC40 × P1(ES1481) |
| SMR2597 | FC40 Δ(xseA-gua) zff-3139∷Tn10kan | Lab collection |
| SMR4251 | FC40 dam13∷Tn9 | FC40 × P1(SMR56, a derivative of GM2159) |
| SMR4576 | FC40 upp∷Tn10dtet+1 | This study* |
| SMR4608 | SMR4576 recA∷Tn10dcam | SMR4576 × P1(SMR4610) |
| SMR4610 | FC40 recA∷Tn10dcam | Lab collection |
| SMR4719 | FC40 recD1018 Δ(xseA-gua) zff-3139∷Tn10kan | Lab collection |
| SMR4721 | SMR4576 recD1018 | SMR4719 × P1(SMR4576) |
| SMR4727 | SMR4721 recA∷Tn10dcam | SMR4721 × P1(SMR4610) |
| SMR4733 | SMR4576 ΔrecG263∷kan | SMR4576 × P1(FC526) |
| SMR4758 | SMR4733 recA∷Tn10dcam | SMR4733 × P1(SMR4610) |
| SMR4822 | SMR4576 ΔruvC64∷kan | SMR4576 × P1(GS1481) |
| SMR4823 | SMR4721 ΔruvC64∷kan | SMR4721 × P1(GS1481) |
| SMR5499 | SMR4576 ΔrecG263∷kan rfa-209∷Tn10 pyrE70 | SMR4733 × P1(BW229) |
| SMR5522 | FC29 ΔruvC64∷kan | FC29 × P1(GS1481) |
| SMR5790 | SMR4576 radC102 | SMR5499 × P1(SR1187) |
| SMR5830 | FC40 dinB10 | (23) |
| SMR5969 | FC40 dinB10 Δ(xseA-gua) zff-3139∷Tn10kan | SMR5830 × P1(STL1605) |
| SMR6047 | SMR4576 ruvC53 eda∷Tn10dcam | This study* |
| SMR6048 | SMR4576 radC102 ruvC53 eda∷Tn10dcam | SMR5790 × P1(SMR6047) |
| SMR6049 | SMR4576 dinB10 | SMR5969 × P1(SMR4576) |
| SR1187 | argE3 hisG4 leuB6 Δ(gpt-proA)62 thr-1 thi-1 ara-14 galK2 lacY1 mtl-1 xyl-5 tsx-33 rfbD1 mgl-1 rpsL31 supE44 rac radC102(=recG) | (77, 78) |
| STL1605 | argE3 hisG4 leuB6 Δ(gpt-proA)62 thr-1 thi-1 ara-14 galK2 lacY1 mtl-1 xyl-5 tsx-33 rfbD1 mgl-1 rpsL31 supE44 Δ(xseA-gua)zff- 3139∷Tn10kan | S. T. Lovett (Brandeis Univ., Waltham, MA) |
*
See Materials and Methods for details of construction.