TABLE 2.
Growth and nitrogenase activities of SNT-1 and wild-type R. rubrum grown on different nitrogen sources
Strain (plasmid) | Nitrogen source | Doubling time (h) | Nitrogenase activity (nmol h−1 mg−1)
|
|
---|---|---|---|---|
In vivo | In vitro | |||
S1 | N2 | 10.3 | 2,405 ± 425 | 252 ± 23 |
SNT-1 | N2 | 29.7 | 515 ± 95 | 358 ± 26 |
S1 | NH4+ | 8.4 | ||
SNT-1 | NH4+ | 8.7 | ||
S1 | Glutamate | 9.4 | 1,759 ± 68 | 324 ± 23 |
SNT-1 | Glutamate | 9.0 | 806 ± 13 | 208 ± 18 |
SNT-1 (pSNCfix) | N2 | 13.5 | 2,253 ± 222 | 503 ± 26 |