Figure 8.
KLF8 potentiates Ras-induced transformation of MCF-10A cells. A and B: KLF8 enhances tumorigenecity of MCF-10A cells induced by H-Ras-V12. The 10A-iK8-V12 cells were generated as described in Materials and Methods. Cells of 1 x 106 were injected into the fat pad of nude mice. The mice were fed with the Dox Diet to induce (I) the expression of KLF8 in the tumor cells in vivo. Uninduced (U) KLF8 expression was maintained by feeding the mice with the Control Diet not supplemented with doxycyclin. Tumors formed by the cells were monitored for 7 months before the tumor images were taken (A) and tumor volumes were analyzed by box plot (B). P < 0.01 compared to U group. C: Induction of KLF8 expression led to a decrease in NUMB expression in the tumors. Specimens of the tumors from A & B were prepared for immunohistochemical staining of the expression of KLF8 and NUMB proteins.