Figure 3.

Combinatorial effects of temsirolimus and ixabepilone in RCC. Synergy curves using various concentrations of ixabepilone were used alone and in combination with three fixed concentrations of temsirolimus (0.1 nM, 1 nM, and 10 nM). Drug interactions were analyzed using CalcuSyn® [26] based upon the Chou-Talalay Method, whereas the combination index (CI) =1 indicates an additive effect, <1 is synergy and >1 is antagonism [27]. Dose curves of A. A498, B. Caki-1, C. Caki-2 and D. UMRC3 overall indicate that temsirolimus and ixabepilone are synergistic when combined as shown by Fa-CI plots. The legend is included and dose ratios that did not exhibit synergy (>1) are marked by black circles.