Figure 5.

Effects of temsirolimus and ixabepilone on RCC protein expression. Western blot panel of 4 RCC cell lines treated with 10 nM temsirolimus (Tem) and 2 nM ixabepilone (Ixa) for 24 hours. PARP and proteins downstream of p-mTOR were examined. All cell lines show increased PARP cleavage, indicative of apoptosis, with the combinatorial treatment (T/I). p-p70s6K expression was decreased in response to Tem with no effects on p4EBP1. Proteins for 4EBP1 were run on a 18% tris-glycine gel for 1.5 hrs at 175 V.