Fig. 4.

Formation of double FA rings requires actin and myosin-dependent contractility. (A) rAEC were plated onto star micropatterned substrates and treated 24 hours later with various cytoskelon-disrupting drugs as indicated, before staining for vinculin and actin. ‘Vehicle’ indicates cells treated with DMSO alone. (B) Cells treated with vehicle, cytochalasin D and blebbistatin were also stained for keratin and talin. For A and B overlays of the two left columns of images are shown in the third column and include DAPI staining of nuclei. Scale bars: 20 µm.