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. 2013 Aug 15;126(16):3746–3755. doi: 10.1242/jcs.128975

Fig. 5.

Fig. 5.

The cFAs within the double ring exhibit faster turnover than pFAs following fluorescence recovery after photobleaching. (A) rAEC both with and without the double ring FA pattern as well as RLE-6TN cells expressing GFP–paxillin were subjected to FRAP (bleached areas indicated with arrows in zoomed images designated with white box in whole cell pre-bleach images). Images of the cells were collected every 10 seconds following photobleaching to monitor the recovery of the fluorescence signal. (B) Fluorescence recovery occurs faster in cFAs in rAEC with the double ring pattern. FRAP data were quantified for five to seven cells per type, with two to six FAs bleached per cell. Data are the average fluorescence recovery values for each time point. (C) Selected time points from the data presented in B were plotted to show the average fluorescence recovery (± s.d.) in each cell type; *P<0.05. Scale bars: 20 µm in pre-bleach; 10 µm in zoom.