FIG. 5.

PILEUP analysis of the partial central domains of 27 P. aeruginosa strains. Amino acid (aa) sequences from amino acids 228 to 297 of a-type flagellins of 24 P. aeruginosa strains analyzed in this study, one a-type strain obtained from R. A. Ansorg, and two complete a-type flagellin sequences already present in the database were aligned by using the PILEUP program of the GCG Wisconsin package. Amino acid substitutions at positions 237, 240, 241, 249, 250, 253, 266, 273, 283, 285, 287, 288, and 289 and two small deletions of 3 and 4 amino acids at positions 254 and 279, respectively, are highlighted in different shades of gray. The A2 flagellin of strain DSM 1128 was peculiar in having additional amino acid substitutions (shown in bold italics).