TABLE 1.
Strains, plasmids, and primers used
| Strain, plasmid, or primer | Description, origin, or sequence | Source, reference, or description |
|---|---|---|
| P. aeruginosa strains | ||
| PAK | Wild-type laboratory strain | D. Bradley |
| PAO1 | Wild-type laboratory strain | P. Phibbs |
| 170002 | Clinical isolate | R. Ansorg |
| ATCC 15691 | B. W. Holloway | 23 |
| DSM 1128 | Ear infection | 23 |
| JJ692 | UTI isolate | S. Lory |
| S54485 | UTI isolate | S. Lory |
| X24509 | UTI isolate | S. Lory |
| UDL | UTI isolate | S. Lory |
| 0252509 | UTI isolate | R. Jones |
| 043618 | UTI isolate | R. Jones |
| 0551701 | UTI isolate | R. Jones |
| 0031261 | UTI isolate | R. Jones |
| 025669 | UTI isolate | R. Jones |
| 0572 | UTI isolate | R. Jones |
| 0581854 | UTI isolate | R. Jones |
| 01991 | UTI isolate | R. Jones |
| S35004 | Blood isolate | S. Lory |
| X13273 | Blood isolate | S. Lory |
| CS2 | Blood isolate | This study |
| B1 | Blood isolate | This study |
| H21651 | Blood isolate | S. Lory |
| S29712 | Blood isolate | S. Lory |
| CF18 | CF isolate | S. Lory |
| CF5 | CF isolate | J. Burns |
| CF27 | CF isolate | J. Burns |
| CF127 | CF isolate | S. Lory |
| CF262 | CF isolate | R. Jones |
| CF282 | CF isolate | R. Jones |
| CF304 | CF isolate | R. Jones |
| CF4 | CF isolate | M. Vasil |
| CF283 | CF isolate | R. Jones |
| CF265 | CF isolate | R. Jones |
| CF280 | CF isolate | R. Jones |
| CF29 | CF isolate | M. Vasil |
| Plasmids | ||
| PCR2.1-TOPO | TA cloning vector, ampicillin resistance, kanamycin resistance | Invitrogen, Inc. |
| PCR2.1AV1190 | PCR2.1-TOPO vector carrying 6.4-kb PCR fragment from JJ692 containing orfA to orfG | This study |
| PCR2.15KBΔGI | PCR2.1-TOPO vector carrying 5.1-kb PCR fragment from JJ692 containing orfH and orfN | This study |
| Primers | ||
| RER142 | GCGAATGGTTGTTGCCGGGCAGGATT | 5′ primer for GI |
| RER144 | GGTCGGCATGACGATGCTGACGAGAG | 3′ primer for GI |
| RER150 | ATGCGAACTTTAGGGGTGCCTGGGCG | 5′ primer for flagellin |
| RER170 | TCGGAAGAAGACGCCGAGCCGAACAG | 3′ primer for flagellin |
| RER154 | GGCACCGTCGACATCGCGAT | Primer for sequencing variable domains of flagellins |
| RER171 | GGCTACTGTGGCATCGCCGACGAAAC | Primer for sequencing GI from CF5 |
| RER172 | GTTTCGTCGGCGATGCCACAGTAGCC | Primer for sequencing GI from CF5 |
| RER166 | CCCAAAGAGCTCGTTTGCCTGAGCTT | Primer for sequencing GI from JJ692 |
| RER167 | GAACTCATGCCCGATGGGGCACGCAT | Primer for sequencing GI from JJ692 |
| AV1 | AAACCCGAATTCTACGCCGGTGTCAGCGTGACGATCGA | Primer for sequencing GI from JJ692 |
| RER190 | GTTTCCCGCCATCATCGACAGCTCCC | Primer for sequencing GI from JJ692 |