Fig. 5.

Induction of ${\text{HCO}}_3^{-}$-rich choleresis by INT-767 depends on Fxr, but not Tgr5. Bile flow and biliary ${\text{HCO}}_3^{-}$ output were measured in Fxr^+/+ and Fxr^−/− mice after 7 days of feeding on a chow or INT-747-, INT-777-, or INT-767-supplemented diet. (A) INT-767 induced bile flow only in Fxr^+/+ mice, whereas INT-747 and INT-777 did not change bile flow in both Fxr^+/+ and Fxr^−/− mice. (B) Only INT-767 induced biliary ${\text{HCO}}_3^{-}$ output in Fxr^+/+, but not in Fxr^−/−, mice. Means of 4–5 mice/group ± SD are presented. *P < 0.05 INT-767-fed versus chow-fed Fxr^+/+ mice (Co). ^#P < 0.05 Fxr^−/− versus Fxr^+/+ mice. Bile flow (C) and biliary ${\text{HCO}}_3^{-}$ (D) output were measured in age-matched Tgr5-Tg and Tgr5^+/+ female mice. Bile flow, as well as biliary ${\text{HCO}}_3^{-}$ output, was undistinguishable between Tgr5-Tg and Tgr5^+/+ mice. Means of 4–5 mice/group ± SD. are presented.