Fig. 1. Myospheroid is required for proper hemocyte migration.

(A) Outline of the MARCM protocol in hemocytes. Cross between DEMON males and FRT19a control or mys¹ mutant virgin female flies for MARCM analysis. (B) Crosses are placed at 25°C for 24 hours. The progeny is submitted to three 1 hour heat-shocks (indicated by arrows) at 37°C before selection of 3^rd instar females containing GFP-expressing hemocytes. (C) Movement of wild-type and mys¹ GFP-expressing hemocytes in 3 to 4 hour APF flies, tracked for 12 minutes (1 min time-lapse interval). Arrowheads indicate filopodium-like protrusions produced by both wild-type and mys¹ mutant hemocytes. Scale bars: 10 µm. (D) Graph showing individual mean cell speed for wild-type and mys¹ clones (2 to 4 hours APF). (E) Graph showing average mean cell speeds for yv control and mys (valium 20) RNAi-expressing flies (between 3 to 4 hours APF). Mann–Whitney tests for non-Gaussian populations were used. Black lines indicate the samples' means; Error bars  =  standard deviation; P-values shown above tested groups.