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. 2013 Jun 26;2(8):838–844. doi: 10.1242/bio.20134374

Fig. 3. SAP130 does not regulate the function of Cul2 and Cul3-based CRLs.

Fig. 3.

(A) NRF-2 was co-transfected with SAP130 or empty vector. Cells were treated with 40 µM CHX and lysed at time 0 h, 2 h, 4 h, 6 h. Lysates were analyzed for NRF-2 degradation rate. (B) SAP130-HA-transfected cells were incubated at 1% oxygen using a Pro-ox oxygen controller and Pro-ox in vitro chamber (BioSpherix, Redfield, NY) for 4 hours to stabilize HIF-1α. Cells were lysed before incubation as a normoxia control and upon re-oxygenation at 0 min, 5 min, 15 min and 20 min.