FIG. 2.

IS1 transposase (InsAB′) protein in hns⁺ and Δhns cells. Cultures of MC1061 strains carrying no IS1 sequence (−), wild-type insAB′ (A6C), or fused-frame insAB′ (GA2GA3C) under araC-araBADp control, on plasmids pDAD18, pCST200, and CST400, respectively, were grown in LB medium at 30°C to late log phase. Portions of the cultures were then added to arabinose (final concentration, 1%) or left untreated (−), and incubation was continued for 1 or 2 h, as shown, before sampling for analysis of the total protein content by SDS-PAGE (12% acrylamide) and Coomassie blue staining. The arrow indicates the position of the InsAB′ band.