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On-line derivatization of 10 μM of glutamate (E) and aspartate (D). Injection times (1, 0.1, and 0.07 s) were optimized for baseline separation of the analytes. Separation buffer: 20 mM borate, 10 mM SDS, pH 9.2. On-line derivatization was performed with 7 mM NDA in ACN, 10 mM NaCN in 50 mM borate buffer at pH 9.2. The separation potential was 500 V/cm.
