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On-line derivatization with concentration change of glutamate (E) demonstrating the rise time (8–13 min) of approximately 5 min. Separation buffer: 20 mM borate, 10 mM SDS, pH 9.2; separation potential 500 V/cm. Derivatization was performed with 7 mM NDA in ACN, 10 mM NaCN in 50 mM borate buffer at pH 9.2.
