TABLE 1.
E. coli K-12 strains, bacteriophage, and plasmids used in this study
| Strain, phage, or plasmid | Relevant genotype | Reference or source |
|---|---|---|
| Strains | ||
| MC4100 | F−araD139 Δ(argF-lac)U169 rpsL150 relA1 flbB5301 deoC ptsF25 rbsR | 20 |
| JM109 | recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 Δ(lac-proAB)/F′ traD36 proAB+lacIqlacZΔM15 | Promega |
| ECL 5001 | MC4100 but Φ(cydA′-lacZ) | 20 |
| ECL 5003 | MC4100 but Δfnr::Tn9(Cmr) Φ(cydA′-lacZ) | 20 |
| ECL 5005 | φ(cydA′-lacZ) Δfnr::Tn9(Cmr) ΔarcB::Kanr | 20 |
| ECL 5201 | φ(cydA′-lacZ) ΔldhA | This study |
| ECL 5202 | φ(cydA′-lacZ) ΔldhA Δdld | This study |
| ECL 5203 | φ(cydA′-lacZ) ΔldhA Δdld Δfnr::Tn9(Cmr) | This study |
| ECL 5204 | φ(cydA′-lacZ) ΔldhA Δdld Δfnr::Tn9(Cmr) ΔarcB::Kanr | This study |
| ECL 5205 | φ(cydA′-lacZ) ΔldhA Δdld Δfnr::Tn9(Cmr) arcBcyt::Kanr | This study |
| P1vir | Laboratory stock | |
| Plasmids | ||
| pKO3 | 21 | |
| pLDH2 | ΔldhA in pKO3 | This study |
| pDLD2 | Δdld in pKO3 | 26 |
| pABW | arcB+ in pBluescript KS II(+) | 20 |
| pABS | arcB78-778 in pBluescript KS II(+) | 20 |
| pIBW | arcB+::Kanr in pIB3 | 20 |
| pIBcyt | arcBΔ23-77::Kanr in pIB3 | This study |