TABLE 2.

Accumulation of d-lactate against concentration gradient by the ΔldhA Δdld mutant strain ECL 5203

Culture conditionsa	Incubation time (min)	Concn (μM)b
+O2	0.5	23.3
	5	67.5
−O2	0.5	8.7
	5	23.7

^aCells were grown in xylose minimal media to an OD₆₀₀ of ∼0.3.

^bd-Lactate uptake was assayed with 4 μM d-[¹⁴C]lactate as the substrate as previously described (27).