TABLE 2.
Complementation of lethal expression of PasB toxin by antidote PasA carried in transa
| Resident plasmid in E. coli CSH50-Iq | No. of transformants per ng upon transformation with:
|
|||||
|---|---|---|---|---|---|---|
| pKK223-3 (control)
|
pTac-FC2pasB
|
pTac-F14pasB
|
||||
| −IPTG | +IPTG | −IPTG | +IPTG | −IPTG | +IPTG | |
| pF14-pasAB (Km) | ∼1,000 | ∼1,000 | ∼1,000 | NG | ∼700 | ∼700 |
| pF14-pasΔAB | ∼1,000 | ∼1,000 | ∼1,000 (sick) | NG | NG | NG |
| pFC2-pasABC | ∼1,000 | ∼1,000 | ∼1,000 | ∼1,000 | ∼500 | NG |
| pFC2-pasABC* | ∼1,000 | ∼1,000 | ∼1,000 | ∼1,000 | ∼500 | NG |
| pFC2-ΔpasABC | ∼1,000 | ∼1,000 | ∼1,000 (sick) | NG | NG | NG |
a
pTC-F14 pasAB and the deletion thereof were carried on plasmids pF14-pasAB (Km) and pF14-pasΔAB, respectively. pTF-FC2 pasABC and pasAB and deletions thereof were carried on plasmids pFC2-pasABC, pFC2-pasABC*, and pFC2-ΔpasABC, respectively. pKK223-3 is the tac promoter fusion vector containing no insert. pTac-FC2pasB contains the pasB of pTF-FC2 under control of the tac promoter. pTac-F14pasB contains the pasB of pTC-F14 under control of the tac promoter. IPTG (1 mM) was used to induce the tac promoter (−, without; +, with). Colony numbers on plates after 24 h of incubation at 30°C are given. NG, no growth.