TABLE 5.
Competitive and noncompetitive plasmid stability in the absence of antibiotic selectiona
| Host cell | pTC-F14-based plasmid and antibiotic marker | pTF-FC2-based plasmid and antibiotic marker | Plasmid retention (%) within cells after 100 generations minus antibiotic selection for:
|
|
|---|---|---|---|---|
| pTC-F14-based plasmids | pTF-FC2-based plasmids | |||
| E. coli CSH50 | pF14-pasAB (Cm) | 99.3 (1.2) | ||
| pF14-pasAB (Km) | 99.7 (0.6) | |||
| pF14-pasΔAB (Km) | 26.6 (29) | |||
| pF14-pasA*B* (Km) | 5.3 (9.2) | |||
| pFC2-pasABC (Km) | 99.0 (1.7) | |||
| pFC2-pasAB*C (Km) | 60.3 (15.4) | |||
| pFC2-pasABC* (Km) | 99.7 (0.6) | |||
| pFC2-ΔpasABC (Km) | 80.7 (14) | |||
| pF14-pasAB (Cm) | pFC2-pasABC (Km) | 1.6 (2.8) | 98.0 (3.5) | |
| pF14-pasAB (Cm) | pFC2-pasAB*C (Km) | 17.0 (5.6) | 91.0 (5.6) | |
| pF14-pasAB (Cm) | pFC2-pasABC* (Km) | 0.6 (1.2) | 89.6 (5.8) | |
| pF14-pasAB (Cm) | pFC2-ΔpasABC (Km) | 75.3 (18) | 89.3 (13.6) | |
a
All values are normalized to 100% at 0 generations. The percentage of plasmid retention is shown as the average of the results from at least three experiments. Standard deviations are shown in parentheses.