TABLE 1.
B. subtilis strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| JH642 | trpC2 pheA1 | J. A. Hoch |
| LAB2135 | trpC2 pheA1 ΔresDE::tet | 52 |
| ORB4599 | trpC2 pheA1 amyE::Pspank-hy resD (pMMN546) | This study |
| ORB4600 | trpC2 pheA1 amyE::Pspank-hy resD (D57A) (pMMN547) | This study |
| ORB4605 | trpC2 pheA1 ΔresDE::tet amyE::Pspank-hy resD (pMMN546) | This study |
| ORB4606 | trpC2 pheA1 ΔresDE::tet amyE::Pspank-hy resD (D57A) (pMMN547) | This study |
| ORB4612 | trpC2 pheA1 ΔresDE::tet amyE::Pspank-hy resD (pMMN546) SPβc2del2::Tn917::pMMN288 (fnr-lacZ) | This study |
| ORB4613 | trpC2 pheA1 ΔresDE::tet amyE::Pspank-hy resD (pMMN546) SPβc2del2::Tn917::pMMN392 (nasD-lacZ) | This study |
| ORB4614 | trpC2 pheA1 ΔresDE::tet amyE::Pspank-hy resD (pMMN546) SPβc2del2::Tn917::pML107 (hmp-lacZ) | This study |
| ORB4616 | trpC2 pheA1 ΔresDE::tet amyE::Pspank-hy resD (D57A) (pMMN547) SPβc2del2::Tn917::pMMN288 (fnr-lacZ) | This study |
| ORB4617 | trpC2 pheA1 ΔresDE::tet amyE::Pspank-hy resD (D57A) (pMMN547) SPβc2del2::Tn917::pMMN392 (nasD-lacZ) | This study |
| ORB4618 | trpC2 pheA1 ΔresDE::tet amyE::Pspank-hy resD (D57A) (pMMN547) SPβc2del2::Tn917::pML107 (hmp-lacZ) | This study |
| Plasmids | ||
| PDR111 | Integration plasmid with Pspank-hy, Ampr Spcr | 10 |
| PTYB4 | Expression vector with self-cleavable intein tag | New England Biolabs |
| pXH22 | pTYB2 carrying resD | 69 |
| pMMN424 | pTYB4 carrying resE | 49 |
| pMMN539 | pTYB4 carrying resD (D57A) | This study |
| pMMN546 | pDR111 carrying resD | This study |
| pMMN547 | pDR111 carrying resD (D57A) | This study |