TABLE 1.
B. subtilis strains and plasmids used
| Plasmid or strain | Relevant characteristic(s) | Origin or reference |
|---|---|---|
| Plasmids | ||
| pDH6 | spoIIQ-gfpmut1 neo in pGEM-T vector (Promega) | 14 |
| pDH8 | thrC::spoIIQ-gfpmut1 | This study |
| pDH9 | spoIIE-uvGFP | This study |
| Strains | ||
| BR151 | trpC2 metB10 lys-3 | Laboratory stock |
| SL10206 | trpC2 metB10 lys-3 spoIIQ::pDH6b,c | |
| SL10257 | trpC2 metB10 lys-3 thrC::spoIIQ-gfpmut1 | This study |
| SL10260 | trpC2 metB10 lys-3 spoIIIE::spc thrC::spoIIQ-gfpmut1 | This study |
| SL10566 | trpC2 metB10 lys3 spoIIQ::pDH6b,c | This study |
| SL11811 | trpC2 metB10 lys-3 spoIIE::pDH9 spoIIQ::pDH6b | This study |
| SL11812 | trpC2 metB10 lys-3 spcaspoIIEV697A::pDH9bspoIIQ::pDH6b | This study |
| SL11818 | trpC2 metB10 lys-3 spcaspoIIEV697A spoIIQ::pDH6b | This study |
| SL11939 | trpC2 metB10 lys-3 spoIIGB::erm spcaspoIIEV697A::pDH9bspoIIQ::pDH6b | This study |
| SL11940 | trpC2 metB10 lys-3 spoIIGB::erm spoIIE::pDH9bspoIIQ::pDH6b | This study |
| SL11954 | trpC2 metB10 lys-3 spoIIGB::erm spoIIQ::pDH6b | This study |
| SL11955 | trpC2 metB10 lys-3 spoIIGB::erm spcaspoIIEV697A spoIIQ::pDH6b | This study |
| SL11975 | trpC2 metB10 lys-3 spoIIIE36 thrC::spoIIQ-gfpmut1 | This study |
| SL11978 | trpC2 metB10 lys-3 spoIIQ::pDH6bspoIIIE::spc | This study |
| SL11979 | trpC2 metB10 lys-3 spoIIQ::pDH6bspoIIIE36 | This study |
| SL12003 | trpC2 metB10 lys-3 spoIIGB::neo spoIIIE::spc spoIIQ::pDH6b | This study |
| SL12005 | trpC2 metB10 lys-3 spoIIGB::neo spoIIQ::pDH6b | This study |
| SL12035 | trpC2 metB10 lys-3 spoIIP::tet spoIIIE::spc spoIIQ::pDH6b | This study |
| SL12065 | trpC2 metB10 lys-3 spoIID::neo spoIIP::tet spoIIIE::spc spoIIQ::pDH6b | This study |
| SL12070 | trpC2 metB10 lys-3 spoIID::neo spoIIIE::spc spoIIQ::pDH6b | This study |
a
Gene is directly upstream of spoIIE.
b
Plasmid has been inserted by Campbell (single-crossover) integration.
c
SL10206 and SL10566 have different resistance genes associated with pDH6: erm and cat, respectively.