TABLE 2.
Compartmentalization of σF activity in spoIIIE mutant strains
| Strain | spoIIIE allele | Location of spoIIQ-gfpa | % Expressionb | Pattern of GFP fluorescencec
|
|||
|---|---|---|---|---|---|---|---|
| % Prespore | % Mother cell | % Prespore and mother cell | % Aseptate cell | ||||
| SL10566 | Wild type | spoIIQ (PS) | 72 ± 18 | 100 | 0 | 0 | 0 |
| SL10257 | Wild type | thrC (MC) | 35 ± 7.0 | 98 ± 3.5 | 0 | 2.0 ± 1.7 | 0 |
| SL11979 | spoIIIE36 | spoIIQ (PS) | 55 ± 13 | 95 ± 2.3 | 0 | 4.7 ± 2.3 | 0 |
| SL11975 | spoIIIE36 | thrC (MC) | 0.67 ± 1.2 | ND | ND | ND | ND |
| SL11978 | spoIIIE::spc | spoIIQ (PS) | 19 ± 1.2 | 7.3 ± 6.1 | 0 | 85 ± 7.6 | 8.0 ± 2.0 |
| SL10260 | spoIIIE::spc | thrC (MC) | 13 ± 3.1 | 1.3 ± 1.2 | 2.3 ± 1.3 | 88 ± 9.2 | 9.3 ± 11 |
a
MC indicates a mother cell, and PS indicates a prespore. In spoIIIE+ strains, thrC is initially in the mother cell but is transferred to the prespore.
b
Percentage of the total population expressing the GFP fusion.
c
The pattern of fluorescence was determined for cells expressing GFP 6 h after the initiation of sporulation in MSSM. Septa were visualized by staining with FM4-64. The data are the means of results from three independent experiments and show the standard deviations (with the qualification that the maximum obtained was 100% and the minimum obtained was 0%). Fifty cells were scored in each experiment. ND, not determined.