TABLE 3.
Compartmentalization of σF activity in strains with mutant spoIIIE and spoIIGB alleles
| Strain | Relevant genotype | Pattern of GFP fluorescencea
|
|||
|---|---|---|---|---|---|
| % Prespore
|
% Prespore and mother cell | % Aseptate cell | |||
| Monosporic | Disporic | ||||
| SL12005 | spoIIGB::neo wild-type spoIIIE | 54 ± 6.9 | 43 ± 6.1 | 0.67 ± 1.2 | 2.7 ± 1.2 |
| SL12003 | spoIIGB::neo spoIIIE::spc | 26 ± 4.0 | 65 ± 7.6 | 4.7 ± 3.1 | 4.0 ± 5.3 |
a
The pattern of fluorescence was determined for cells expressing GFP 6 h after the initiation of sporulation in MSSM. Septa were visualized by staining with FM4-64. The data are the means of results from three independent experiments and show the standard deviations (with the qualification that the maximum obtained was 100% and the minimum obtained was 0%). Fifty cells were scored in each experiment.