TABLE 4.
Compartmentalization of σF activity in strains with different spoIIIE spoIID and spoIIP alleles
| Strain | Relevant genotype | Pattern of GFP fluorescencea
|
||
|---|---|---|---|---|
| % Prespore | % Prespore and mother cell | % Aseptate cell | ||
| SL12035 | spoIIP::tet spoIIIE::spc | 71 ± 11 | 28 ± 11 | 0.67 ± 1.2 |
| SL12070 | spoIID::neo spoIIIE::spc | 88 ± 6.9 | 12 ± 6.9 | 0 |
| SL12065 | spoIID::neo spoIIP::tet spoIIIE::spc | 99 ± 2.3 | 0.67 ± 1.2 | 0.67 ± 1.2 |
a
The pattern of fluorescence was determined for cells expressing GFP 6 h after the initiation of sporulation in MSSM. Septa were visualized by staining with FM4-64. The data are the means of results from three independent experiments and show the standard deviations (with the qualification that the maximum obtained was 100% and the minimum obtained was 0%). Fifty cells were scored in each experiment.