TABLE 5.
Compartmentalization of σF activity in strains with different spoIIE and spoIIGB alleles
| Strain | Relevant genotype | Pattern of GFP fluorescencea
|
||
|---|---|---|---|---|
| % Prespore | % Prespore and mother cell | % Aseptate cell | ||
| SL10206 | Wild-type spoIIE wild-type spoIIGB | 100 | 0 | 0 |
| SL11954 | Wild-type spoIIE spoIIGB::erm | 100 | 0 | 0 |
| SL11811 | spoIIE-uvgfp wild-type spoIIGB | 99 ± 1.2 | 0.67 ± 1.2 | 0 |
| SL11940 | spoIIE-uvgfp spoIIGB::erm | 99 ± 1.2 | 0 | 0.67 ± 1.2 |
| SL11818 | spoIIEV697A wild-type spoIIGB | 6.0 ± 3.5 | 8.0 ± 2.0 | 86 ± 4.0 |
| SL11955 | spoIIEV697A spoIIGB::erm | 2.7 ± 4.6 | 13 ± 4.2 | 85 ± 1.2 |
| SL11812 | spoIIEV697A-uvgfp Wild-type spoIIGB | 66 ± 10 | 4.0 ± 3.5 | 30 ± 9.2 |
| SL11939 | spoIIEV697A-uvgfp spoIIGB::erm | 28 ± 11 | 31 ± 6.1 | 41 ± 5.0 |
a
The pattern of fluorescence was determined for cells expressing GFP 6 h after the initiation of sporulation in MSSM. Septa were visualized by staining with FM4-64. The data are the means of results from three independent experiments and show the standard deviations (with the qualification that the maximum obtained was 100% and the minimum obtained was 0%). Forty-nine or 50 cells were scored in each experiment.