FIG. 4.

(A) Structure and comparison of expression profile in a predicted fatty acid synthesis operon (CAC3568 to CAC3579). The gene identified as CAC3580 (annotated as related to 2-nitropropane dioxygenase) was omitted from analysis for missing too many data points. The fatty acid synthesis pathway is based on the work of de Mendoza et al. (8) and the Kyoto Encyclopedia of Genes and Genomes (KEGG; www.genome.ad.jp/kegg/kegg2.html). P, predicted promoter; ACP, acyl carrier protein; R-ScoA, straight- or branched-chain acyl-CoA primer. (B) Structure and comparison of primary metabolism expression profiles in C. acetobutylicum spo0A strains as generated by microarrays. The last column indicates whether differential expression was achieved in each experiment to a 95% confidence interval on at least one array for at least two time points; an asterisk before the slash indicates differential expression in the wild-type (WT)/SKO1 experiment, and an asterisk after the slash indicates differential expression in the 824(pMSPOA)/824(pIMP1) experiment. The colorimetric ratio is on the same scale as in Fig. 3, and the yellow triangle represents the direction of increasing culture time.