FIG. 2.

Survival of the dnaE74(Ts) strain and its suppressor strains at 40°C. Strains were incubated overnight in LB medium containing streptomycin at 40°C. The cultures were then washed, resuspended in LB medium, plated on prewarmed LB medium plates, and incubated at 40°C for different times. The plates were then incubated for 24 h at 27°C, and the organisms were counted. No colonies were visible at the time of the switch from 40 to 27°C. N, viable count after incubation; No, viable count at zero time.