TABLE 1.
Strains used
| Identifying genotype of strain | Characteristics | Reference(s) and/or source |
|---|---|---|
| BS40 (dnaE+) | metB metD Δ(proAB lac) rpsL (Smr) | 36 |
| dnaE74 (BS40dnaE74) | metB metD Δ(proAB lac) dnaE74 rpsL (Smr) | 35 |
| dnaE486 Tn10 | dnaE486 zae502::Tn10 | Roger Woodgate |
| dnaE486 | thr-1 leuB6(Am) fhuA21 dnaE486(Ts) lacY1 glnV44 λ−rfbD1 thyA6 rpsL67 thi-1 deoC1 met-89 | E. coli Genetic Stock Center (39) |
| BS40 dnaE486 Tetr | metB metD Δ(proAB lac) rpsL (Smr) dnaE486::Tn10 | From dnaE486 Tn10 strain by transduction |
| BS40 dnaE486 | metB Δ(proAB lac) rpsL (Smr) dnaE486 | From dnaE486 strain by transduction |
| CGSC 7027 ΔrecA (Camr) | λ−recA938::Tn9-200 recD1014 (Nuc−) hsdR2 mdoB202::Tn10 | E. coli Genetic Stock Center (40) |
| BS40 dnaE74 ΔrecA | metB Δ(proAB lac) dnaE74(Ts) rpsL (Smr) ΔrecA (Camr) | By transduction from ΔrecA strain |
| RW82 Δumu | uvrA6 thi-1 thr-1 araD-14 leuB6 lacY1 ilv323(Ts) Δ(gpt-proA)62 mtl-1 xyl-5 rpsL31 tsx-33 supE44 galK2(oc) hisG4(oc) rfbD1 kdgK51 sulA211 Δ(umuDC)595::cat | Roger Woodgate (41) |
| CGSC 7699 ΔsulA | Δ(gpt-lac)5 tsx-35 λ−ompF626 zcb-222::Tn10 pyrD34 or pyrD36 sulA3 e14-rac-0 rfbD1 mg1-51 recA441(Ts) relA1 rpsL31 (Strr) kdgK51 xylA5 mtl-1 spoT1 thy-1 creC510 | E. coli Genetic Stock Center (27) |
| dnaE74 ΔsulA | metB (ΔproAB lac) rpsL (Smr) dnaE74 ΔsulA Tn10 | |
| CGSC7703 ΔrecQ | λ− IN(rrnD-rrnE)1 rph-1 recQ1803::Tn3 | E. coli Genetic Stock Center (28) |
| dnaE74 ΔrecQ | metB Δ(proAB lac) rpsL (Smr) dnaE486 recQ1803::Tn10 | From CGSC 7703 by transduction |
| CGSC 7351 | nadA57::Tn10 λ−rph-1 | E. coli Genetic Stock Center (34) |
| dnaE74 cydA | metB Δ(proAB lac) rpsL (Smr) dnaE74 cydA | This study |
| dnaE74 cydA Tetr | metB Δ(proAB lac) rpsL (Smr) dnaE74 cydA nadA57::Tn10 | By transduction with P1 phage grown on CGSC 7351 |
| dnaE+cydA Tetr | metB metD Δ(proAB lac) rpsL (Smr) cydA nadA57::Tn10 | From BS40 by transduction with P1 phage grown on dnaE74 cydA Tetr cells |
| dnaE74 cydA Tetr (stock) | metB Δ(proAB lac) rpsL (Smr) dnaE74 cydA nadA57::Tn10 | By transduction with P1 phage grown on dnaE+cydA Tetr cells |
| dnaE486 cydA Tetr | metB Δ(proAB lac) rpsL (Smr) dna486 cydA nadA57::Tn10 | From BS40 dnaE486 strain by transduction with P1 grown on dnaE74 cydA Tetr cells |
| dnaE74/pBR322 | metB Δ(proAB lac) dnaE74(Ts) rpsL/pBR322 | By CaCl2 transformation |
| dnaE74/pTK1 | metB Δ(proAB lac) dnaE74(Ts) rpsL/pTK-1 | By CaCl2 transformation with plasmid from R. Gennis (16, 22) |
| BS40/pTK1 | metB Δ(proAB lac) rpsL (Smr)/pTK-1 | By CaCl2 transformation with plasmid from R. Gennis (16, 22) |