TABLE 7.
Wild-type cydA reverses the suppressor effect
| Genotype of strain |
N/N0 with the following plasmida:
|
||
|---|---|---|---|
| None | pBR322 | pTK-1 | |
| dnaE74 cydA | 8.5 × 10−1 | 7.5 × 10−1 | 2.5 × 10−3 |
| dnaE74 | 4.8 × 10−6 | 2.5 × 10−5 | 1.4 × 10−5 |
a
N, viable count after incubation; N0, viable count at zero time. Freshly transformed strains were incubated overnight at 27°C in LB medium or LB medium containing ampicillin for cells with a plasmid. Cultures were then diluted and plated. The plates were incubated either at 27°C or at 40°C for 24 h and then switched to 27°C. Strains were transformed with plasmids by the CaCl2 method (26) and selected on LB medium plates containing ampicillin incubated at 27°C, and colonies were picked and used immediately after transformation.