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In vitro expression of the bphS and bphT genes of Rhodococcus sp. strain RHA1. The bphST and bphT genes in pBAB62 and pBAB14, respectively, were expressed in the E. coli S30 extract system containing [35S]methionine. Labeled polypeptides were separated by SDS-PAGE. The expected bphS and bphT products are indicated by arrowheads. Lane 1, 14C-labeled molecular weight marker (Pharmacia); lanes 2, 3, and 4, gene products of pBSL (vector control), pBAB62, and pBAB14, respectively.
