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Expression of clpP and clpL genes under stress conditions. Total RNAs were isolated from O. oeni cells grown at 30°C (lane 1) and submitted to heat shock (30 min at 42°C) (lane 2) or ethanolic shock (30 min at 10% [vol/vol]) (lane 3). Northern blot analyses of RNAs were performed with clpP probe (A); clpL probe (B); or a mixture of clpP, clpL, and intergenic region-specific probes (C). The sizes of the transcripts detected are indicated. In all cases, 15 μg of RNA was applied per lane.
