FIG. 5.

Determination of the 5′ end of clp transcripts. The RT reaction was performed with 5 μg of total RNA isolated from O. oeni grown at 30°C or heat shocked for 1 h at 42°C. Lanes A, C, G, and T show the dideoxy sequencing ladder obtained with the same primer as that used for the reverse transcriptase reaction. The asterisk indicates the base representing the 5′ end of clpP mRNA or clpL mRNA in the sequence shown to the left. The RBS is double underlined. The putative −10 and −35 sequences are underlined and in boldface. An asterisk indicates the transcription start site. The ATG start codon was boxed. Arrows indicate the probable operator sites for CtsR. Positions relative to the translation initiation site are indicated to the left. (A) Determination of the 5′ end of the clpP transcript with oligonucleotide Pi2. (B) Determination of the 5′ end of the clpL transcript with oligonucleotide L2.