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. 2013 Sep;92(9):825–832. doi: 10.1177/0022034513497961

Figure 4.

Figure 4.

N-GMSCs require FASL to maintain elevated immunomodulatory function. (A) Western blot analysis showed that N-GMSCs expressed elevated levels of FASL compared with M-GMSCs. (B, C) When co-cultured with T-cells, N-GMSCs showed an elevated capacity to inhibit T-cell viability when compared with M-GMSCs. Apoptosis assay confirmed that N-GMSCs more effectively induced 7AAD/Annexin-positive apoptotic T-cells when compared with the M-GMSC group. (D) Western blot analysis showed efficacy of knockdown of fasl expression by siRNA in N-GMSCs. (E, F) When co-cultured with T-cells, apoptosis assay confirmed that FASL knockdown N-GMSCs resulted in a reduced capacity to induce 7AAD/Annexin-positive apoptotic T-cells when compared with the control siRNA group. **p < .01; ***p < .001; n = 3. Error bars: mean ± SD.