Table 2.
Primer | Template | Vmax, nM/min | Km, μM | Vmax/Km | f |
---|---|---|---|---|---|
G | 3′ T (nondamaged) | 0.66 ± 0.03 | 13 ± 2 | 0.05 | 2.9 × 10−2 |
A | 3′ T (nondamaged) | 0.50 ± 0.02 | 0.29 ± 0.03 | 1.7 | 1.0 |
T | 3′ T (nondamaged) | 0.17 ± 0.01 | 76 ± 21 | 0.002 | 1.2 × 10−3 |
C | 3′ T (nondamaged) | ND | >200 | <0.001 | <6 × 10−4 |
G | 3′ T (TT dimer) | 0.42 ± 0.03 | 5.3 ± 1.9 | 0.079 | 1.4 × 10−1 |
A | 3′ T (TT dimer) | 0.45 ± 0.02 | 0.82 ± 0.08 | 0.55 | 1.0 |
T | 3′ T (TT dimer) | 0.25 ± 0.009 | 130 ± 20 | 0.0019 | 3.5 × 10−3 |
C | 3′ T (TT dimer) | 0.15 ± 0.013 | 120 ± 40 | 0.0013 | 2.4 × 10−3 |
G | 5′ T (nondamaged) | 0.43 ± 0.006 | 8.8 ± 3.3 | 0.048 | 2.7 × 10−2 |
A | 5′ T (nondamaged) | 0.51 ± 0.03 | 0.28 ± 0.04 | 1.8 | 1.0 |
T | 5′ T (nondamaged) | 0.53 ± 0.05 | 76 ± 21 | 0.0070 | 3.9 × 10−3 |
C | 5′ T (nondamaged) | ND | >200 | <0.001 | <6 × 10−4 |
G | 5′ T (TT dimer) | 0.55 ± 0.05 | 49 ± 12 | 0.011 | 7.9 × 10−3 |
A | 5′ T (TT dimer) | 0.49 ± 0.06 | 0.35 ± 0.12 | 1.4 | 1.0 |
T | 5′ T (TT dimer) | 0.078 ± 0.02 | 140 ± 50 | 0.00056 | 4.0 × 10−4 |
C | 5′ T (TT dimer) | 0.37 ± 0.04 | 65 ± 17 | 0.0057 | 4.1 × 10−3 |
Mispair extension was examined in the presence of the next correct nucleotide: dATP for mispairs at the 3′ T and dTTP for mispairs at the 5′ T. ND, not determined.