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. 2001 Jul 17;98(15):8355–8360. doi: 10.1073/pnas.121007298

Table 2.

Kinetics of mispair extension by yeast Pol η on undamaged and TT dimer-containing DNA substrates

Primer Template Vmax, nM/min Km, μM Vmax/Km fInline graphic
G 3′ T (nondamaged) 0.66  ± 0.03 13  ± 2 0.05 2.9  × 10−2
A 3′ T (nondamaged) 0.50  ± 0.02 0.29  ± 0.03 1.7 1.0
T 3′ T (nondamaged) 0.17  ± 0.01 76  ± 21 0.002 1.2  × 10−3
C 3′ T (nondamaged) ND >200 <0.001 <6  × 10−4
G 3′ T (TT dimer) 0.42  ± 0.03 5.3  ± 1.9 0.079 1.4  × 10−1
A 3′ T (TT dimer) 0.45  ± 0.02 0.82  ± 0.08 0.55 1.0
T 3′ T (TT dimer) 0.25  ± 0.009 130  ± 20 0.0019 3.5  × 10−3
C 3′ T (TT dimer) 0.15  ± 0.013 120  ± 40 0.0013 2.4  × 10−3
G 5′ T (nondamaged) 0.43  ± 0.006 8.8  ± 3.3 0.048 2.7  × 10−2
A 5′ T (nondamaged) 0.51  ± 0.03 0.28  ± 0.04 1.8 1.0
T 5′ T (nondamaged) 0.53  ± 0.05 76  ± 21 0.0070 3.9  × 10−3
C 5′ T (nondamaged) ND >200 <0.001 <6  × 10−4
G 5′ T (TT dimer) 0.55  ± 0.05 49  ± 12 0.011 7.9  × 10−3
A 5′ T (TT dimer) 0.49  ± 0.06 0.35  ± 0.12 1.4 1.0
T 5′ T (TT dimer) 0.078  ± 0.02 140  ± 50 0.00056 4.0  × 10−4
C 5′ T (TT dimer) 0.37  ± 0.04 65  ± 17 0.0057 4.1  × 10−3

Mispair extension was examined in the presence of the next correct nucleotide: dATP for mispairs at the 3′ T and dTTP for mispairs at the 5′ T. ND, not determined.