Figure 2.

Sortilin deficiency does not impact α- and β-secretase activities. A, B, Quantitative analysis of C-terminal fragments (CTF) C99 and C83 in total brain extracts of Sort1^+/+ and Sort1^−/− mice on the PDAPP background using Western blotting (A) and densitometric scanning of replicate blots (B; n = 7–11 per genotype). Detection of APP and tubulin served as loading controls in A. C, Activity of BACE1 in mice of the indicate genotypes using FRET assay as described in Materials and Methods (n = 4 per group). RFU, Relative fluorescent unit.