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. 2013 Jan 2;33(1):358–370. doi: 10.1523/JNEUROSCI.2425-12.2013

Figure 6.

Figure 6.

Accumulation of APOE in the brain of sortilin-deficient mice. A, B, Levels of APOE in hippocampus (Hip) and cortex (Ctx) of wild-type (Sort1+/+) and sortilin-deficient (Sort1−/−) mice at 3 months of age were determined by Western blot analyses (A) and quantified by densitometric scanning of the respective membranes (n = 6 per group) (B). In A, detection of tubulin served as loading control. Extracts from mice genetically deficient for APOE (Apoe−/−) were used as negative control for specificity of the APOE immunoreactivity (indicated by arrowheads). A similar difference in APOE levels was also seen in mice at 9 months of age (Sort1+/+, 100.0 ± 10.19%, n = 6; Sort1−/−, 176.1 ± 10.3%, n = 7). C, Quantitative RT-PCR analyses of Apoe mRNA levels in hippocampus and cortex of Sort1+/+ and Sort1−/− animals at 2 months of age (n = 4 per group). All values are mean ± SEM and are given as percentage of the wild-type control (mean set at 100%). Significant differences between genotypes are indicated (**p < 0.01, ***p < 0.001).