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. 2013 Jan 2;33(1):358–370. doi: 10.1523/JNEUROSCI.2425-12.2013

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Copyright © 2013 the authors 0270-6474/13/330358-13$15.00/0

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Figure 7. — Sortilin activity does not impact APOE release from primary astrocytes. APOE was immunoprecipitated from cell lysate and conditioned media of primary cultures of astrocytes of newborn Sort1^+/+ and Sort1^−/− animals. The levels of APOE in lysate and media at the indicated time points were determined by autoradiography (A) and quantified by densitometric scanning of replicate blots (B). APOE levels were normalized with respect to the sample with the highest level of APOE in lysate or media (set at 100%), respectively. Bar graphs in B show mean ± SD value of three separate experiments.