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Sortilin interacts with all human APOE isoforms. A–C, Surface plasmon resonance analyses of ligand binding to sortilin immobilized on the BIAcore chip surface. Binding of APOE-free liposomes and of liposomes complexed with recombinant APOE2 (A), APOE3 (B), and APOE4 (C) was tested in the presence and absence of 20 μm NT. D, Surface plasmon resonance analyses of binding of a concentration series of Aβ40 to sortilin immobilized on the BIAcore chip surface.
