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. Author manuscript; available in PMC: 2014 Apr 29.
Published in final edited form as: Dev Cell. 2013 Apr 29;25(2):196–206. doi: 10.1016/j.devcel.2013.03.017

Table 1. Etv2 Function Is Required at Earlier Stages for Arterial grl Expression and at Later Stages for Venous mrc1 Expression.

grl Expression, 24 hpf Normal Dorsal Aorta Reduced/Fragmented Dorsal Aorta Absent Dorsal Aorta Number of Embryos
Control uninjected 100% 0 0 40
Etv2 MO uncaged at 30% epiboly 0 4% ± 4% 96% ± 4% 30
7-somite 65% ± 15% 35% ± 15% 0 23
13- to 14-somite 92% ± 2% 8% ± 2% 0 24
16- to 17-somite 100% 0 0 23
Never uncaged 100% 0 0 25
mrc1 Expression, 24 hpf Normal Cardinal Vein Reduced Cardinal Vein Absent Cardinal Vein Number of Embryos
Control uninjected 100% 0 0 40
Etv2 MO uncaged at 30% epiboly 0 0 100% 30
7-somite 0 100% 0 30
13- to 14-somite 33% ± 4% 67% ± 4% 0 25
16- to 17-somite 33% ± 8% 67% ± 8% 0 19
Never uncaged 88% ± 9% 12% ± 9% 0 40

Data represent an average from two independent experiments. grl and mrc1 expression analyzed at 24 hpf ± refers to the SE.