Table 1. Etv2 Function Is Required at Earlier Stages for Arterial grl Expression and at Later Stages for Venous mrc1 Expression.
grl Expression, 24 hpf | Normal Dorsal Aorta | Reduced/Fragmented Dorsal Aorta | Absent Dorsal Aorta | Number of Embryos |
---|---|---|---|---|
Control uninjected | 100% | 0 | 0 | 40 |
Etv2 MO uncaged at 30% epiboly | 0 | 4% ± 4% | 96% ± 4% | 30 |
7-somite | 65% ± 15% | 35% ± 15% | 0 | 23 |
13- to 14-somite | 92% ± 2% | 8% ± 2% | 0 | 24 |
16- to 17-somite | 100% | 0 | 0 | 23 |
Never uncaged | 100% | 0 | 0 | 25 |
mrc1 Expression, 24 hpf | Normal Cardinal Vein | Reduced Cardinal Vein | Absent Cardinal Vein | Number of Embryos |
---|---|---|---|---|
Control uninjected | 100% | 0 | 0 | 40 |
Etv2 MO uncaged at 30% epiboly | 0 | 0 | 100% | 30 |
7-somite | 0 | 100% | 0 | 30 |
13- to 14-somite | 33% ± 4% | 67% ± 4% | 0 | 25 |
16- to 17-somite | 33% ± 8% | 67% ± 8% | 0 | 19 |
Never uncaged | 88% ± 9% | 12% ± 9% | 0 | 40 |
Data represent an average from two independent experiments. grl and mrc1 expression analyzed at 24 hpf ± refers to the SE.