Figure 7. Venous-restricted expression of EphB1 in the adult skin.

Whole-mount triple immunofluorescence analysis of adult ear skin from EphB4^taulacZ/+ heterozygous (A, D, G), ephrinB2^taulacZ/+ heterozygous (B, E, H), and wild-type (C, F, I) mice was performed with antibodies to ß-galactosidase (A, G, ß-gal for EphB4^taulacZ, green, open arrows; B, H, ß-gal for ephrinB2^taulacZ, green; open arrows), or LYVE-1 (C, I, green, open arrowheads) in combination with EphB1 (A-I, red, arrowheads) and PECAM-1 (A-F, blue). Venous-restricted expression of EphB1 persists into adulthood. No significant expression of the arterial marker ephrinB2^taulacZ and the lymphatic marker LYVE-1 was detected in EphB1⁺ vessels. Inset in H shows single plane image of the white boxed region, suggesting that some overlapping signals for EphB1 in ephrinB2^taulacZ-expressing vessels is caused by the preparation of compressed z-stack images. Scale bars are 100 μm.