Figure 3. Binding of Sfl1p-HA₃ and Sfl2p-HA₃ to selected target promoters.

Strains sfl1-CaEXP-SFL1-HA₃ (Sfl1p-HA₃) and sfl2-CaEXP-SFL2-HA₃ (Sfl2p-HA₃) together with their respective untagged control strains (Vector) were grown under the same conditions as those for the ChIP-Seq experiment prior to ChIP followed by PCR to detect specific Sfl1p and Sfl2p binding enrichment at selected target promoters (See Materials and Methods for details). PCR was performed using primers corresponding to the promoter region of the indicated genes. The URA3 and YAK1 genes were used as a negative control for ChIP enrichment. Primer efficiency (shown on the right panel) was tested by the ability of the corresponding primers to quantify 10-fold serially diluted whole cell extract DNA (WCE, ChIP input samples, dilution factors are indicated at the top of the right panel).