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. 2013 Aug 15;9(8):e1003693. doi: 10.1371/journal.pgen.1003693

Figure 4. Dissection of QTL3 to identify the causative gene.

Figure 4

(A) Fine-mapping of QTL3 by scoring seven selected SNPs in 62 individual thermotolerant segregants confirms significant linkage with the genome of the inferior parent strain BY4742 of the region between 930,000 and 970,000 bp on chromosome IV. The genes containing at least one non-synonymous mutation within the ORF are indicated with an asterisk. This region has been divided into two fragments for bulk RHA, as indicated. (B) Example of bulk RHA for the block of genes on FRAGMENT1. A pair of reciprocal deletion strains for either FRAGMENT1 or FRAGMENT2 was constructed as shown and tested for growth at high temperature. (C) Identification of the causative gene in QTL3. Bulk RHA shows that FRAGMENT1, derived from BY4742, confers higher thermotolerance compared to FRAGMENT1, derived from 21A, whereas for FRAGMENT2 there was no difference. RHA for the individual genes within FRAGMENT1 identified PRP42 as the causative gene.