Figure 5. Thermotolerance of the downgraded parent strains and their segregants.

(A) Growth at 41°C of the original parent strains, 21A and BY4742, the downgraded parent strains, 21A^DG and BY4742^DG, and hybrid diploids in the four combinations. All strains were spotted on the same plate. (B) Growth at 40.7°C of the original parent strains, 21A and BY4742, the downgraded parent strains, 21A^DG and BY4742^DG, and ten segregants from the hybrid 21A^DG/BY4742^DG. The strain pairs for each gene were always spotted on the same plate.