Figure 2. TRO analysis showing transcription readthrough and alteration in promoter-associated transcription in the clp1^ts mutant.

(A) Schematic depiction of the CHA1 gene indicating the positions of the probes (A–I) used in the TRO assay. (B) TRO analysis of CHA1 in the wild type strain at 25°C and 37°C under induced transcription of the gene. (C) TRO analysis of CHA1 in the temperature-sensitive mutant of Clp1 at the permissive (25°C) and non-permissive (37°C) temperatures. (D) and (E) Quantification of the data shown in B and C respectively. Error bars indicate one unit of standard deviation. The results shown are an average of at least four independent replicates. ST = Serine/Threonine, WT = Wild type, TRO = Transcription run-on.