Figure 5. The promoter occupancy of the general transcription factors is not appreciably affected in the clp1^ts mutant.

(A, C) Schematic depictions of INO1 and CHA1 indicating the position of ChIP primer pairs. (B, D) Quantification of ChIP analysis showing crosslinking of the general transcription factors TFIID, TFIIB, TFIIF, TFIIE and TFIIH to different regions of INO1 and CHA1 in the clp1 mutant at the permissive (25°C, black bars) and non-permissive (37°C, grey bars) temperatures. The results shown are an average of at least six independent PCRs from four separate immunoprecipitates from two independently grown cultures. Error bars indicate one unit of standard deviation.