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. 2013 Aug 15;9(8):e1003696. doi: 10.1371/journal.pgen.1003696

Figure 2. Measurement of reactive oxygen species (ROS) production and mitochondrial DNA copy number.

Figure 2

(A) Flow cytometric measurement of reactive oxygen species in parental 143B cells, B4 and E cybrids after treatment with 0, 200 and 400 uM of bile acid for 24 h. * P<0.05 compared at the same doses of bile acid. (B) Changes of mitochondrial DNA copy number in parental 143B cells, B4 and E cybrids after treatment with 0 or 200 uM bile acid for 6, 24 and 48 h. The results represented were mean ± SE in six times tests * P<0.05 compared to cybrid E+200 uM of bile acid.